refurbished HPLC and UHPLC
Waters ACQUITY UPLC with PDA detector and Column Manager, refurbished
A four-module ACQUITY stack built for method development: binary pump, cooled sample manager, a Column Manager that switches between columns, and a photodiode array detector. The photograph is the actual unit. The PDA is the reason to choose this system over a single-wavelength one. It records the full spectrum from 190 to 500 nm across every peak, so you can choose the wavelength after the run, check peak purity, and compare a peak's spectrum against a reference standard. Peak purity is the usual way to show specificity in a stability-indicating method. Two things to know for spectral work. Keep the peak below 1 AU, because above that the photometric error changes the shape of the spectrum, and Waters says so in its PDA guide. And compare spectra taken at the same mobile phase composition, because pH and solvent change the spectrum too. The Column Manager holds two columns up to 150 mm, or four up to 50 mm, so you can screen columns without re-plumbing. Like any UPLC, it will run your HPLC methods only after checking. The system volume is much smaller, so a gradient method elutes earlier, and moving to smaller particle columns is a change you have to justify under USP <621>. The pump is binary, so methods that mix three or four solvents are out. Tell us what you need to run and we will tell you whether this fits.
- Type
- UPLC (UHPLC), binary gradient
- Pump
- Binary Solvent Manager, high-pressure mixing of two solvents chosen from four (A1/A2, B1/B2), built-in six-channel vacuum degasser, 0.001 to 2.000 mL/min, up to 15,000 psi (1034 bar) at 1 mL/min
- Autosampler
- Sample Manager, 10 µL loop as standard (1 to 50 µL loops available), sample compartment cooled to 4 °C, 2 mL vial or microplate trays
- Column compartment
- Column Manager, holds two columns up to 150 mm (four up to 50 mm), 4 to 90 °C, two switching valves for column selection
Price on request
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