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Independent. Diagnosis first, parts second.

+1 437-505-5995 [email protected]

Chromkit
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Chromatography supplies and diagnosis · Ontario

Before you replace it, check that it is the problem.

Labs replace lamps, columns and cartridges that were never at fault. Chromkit is run by Dr. Nazmul Alam, a PhD in separation science with 14 years on LC-MS and HPLC. Tell us the symptom, or the compound you are trying to extract and separate, and we will tell you what is actually wrong. Sample preparation, separation, detection. We supply the part if you turn out to need one.

Describe the problem +1 437-505-5995 Browse parts
Chromkit Inc.
federally incorporated, Mississauga
14 yrs
founder’s time at the instrument
Importer of record
one Canadian invoice, in CAD
Independent
no service contract to protect

How it works

No charge for the first look. If it turns out you do not need to buy anything, we will say so.

  1. 01

    Send the symptom

    Low recovery, peak fronting, drifting baseline, lost sensitivity, a flash run that will not resolve. Tell us the compound, the matrix, how you extract it and the system. A photo of the chromatogram or the TLC plate helps more than a paragraph.

  2. 02

    Get the diagnosis

    We tell you the two or three places we would look first, and what to check before spending anything. Usually the same working day.

  3. 03

    Order the part, if you need one

    If a part is genuinely the answer, we source it and quote it. If your current column is fine, that is the answer you get.

Describe the problem

What we supply

All chromatography supplies in one place, or skip the diagnosis and go straight to a quote.

Instrument parts 109 listed

Detector lamps and light sources

39

Deuterium, tungsten and xenon lamps for HPLC, UV-Vis and AAS detectors. Cross-referenced to the original part number so you can check it against the one in your instrument.

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Pump and injector spares

48

Plunger rods, piston seals, check valves, rotor seals, needle seats and inlet filters, for Agilent, Shimadzu, Thermo and Waters pumps and autosamplers. The parts behind a drifting baseline, carryover, and a pressure trace that will not settle.

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Spectrophotometer and AAS lamps

22

Deuterium, hollow cathode and element lamps for benchtop spectrophotometers and atomic absorption systems. Grouped by instrument.

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Consumables 27 listed

Sample preparation

10

SPE cartridges and 96-well plates, QuEChERS, protein precipitation, syringe filters, SPME. Our founder's PhD was on extraction, so this is the one category where we would rather you asked before you ordered.

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Capillary electrophoresis consumables

5

Vials, caps, gel buffer and standards for CE systems. Confirm the format against your instrument tray before ordering.

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Flash and preparative columns

4

Silica and bonded-phase flash cartridges, 4 g to 330 g. Sold to order, not from a price list, because the right cartridge depends on your compound and your loading, not on what is in stock.

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HPLC and UHPLC columns

8

Reversed phase, HILIC, chiral, guard columns. Chosen for the analyte, not the catalogue page.

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Instruments 13 listed

LC-MS and LC-MS/MS

1

Triple quadrupole and high resolution systems, used and refurbished, with PM history.

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HPLC and UHPLC

4

Complete stacks or single modules: pumps, autosamplers, detectors, column ovens.

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GC and GC-MS

3

Gas chromatographs and mass selective detectors, with the inlet and detector configuration stated.

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GC autosamplers and modules

5

New aftermarket liquid and headspace samplers and trays, sourced to order. These are not used units. Compatibility is the whole question on a sampler, so tell us the GC model and the software that controls it and we will confirm the fit in writing before quoting.

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Featured

Instrument parts →Consumables →Instruments →
Deuterium lamp for Waters detectors, flanged base with a flying lead and a keyed connector.

Detector lamps and light sources

Deuterium lamp for Waters 2489 and 2998 detectors

Aftermarket deuterium lamp for the Waters 2489 and 2998, cross-referenced to 201000281. The 2489 runs at set wavelengths and the 2998 reads the full spectrum, and the same lamp serves both. On either detector, deuterium output falls fastest in the deep UV, so a method running at 210 nm loses sensitivity long before one running at 254 nm does. This detector expects a lamp carrying an RFID tag. The chip is listed in the specification above, and it is worth confirming before you order, because a chipless lamp can be refused by the instrument.

Type
Deuterium
Fits
Waters 2489, 2998
Replaces
201000281, 201000273
RFID chip
Yes

Price on request

Enquire

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Illustration of a plunger rod and pump seal. Generic drawing, not a photograph of this part. Illustration

Pump and injector spares

PerformancePLUS check valve cartridge for Waters 2695

Check valve cartridge for the Waters 2695 Alliance, cross-referenced to 700000254. A check valve that is not seating gives you a pressure trace that swings with the pump stroke, and the free test is worth running first: sonicate the cartridge in isopropanol and refit it. A valve held open by a particle or by precipitated buffer often recovers, and one that is genuinely worn does not. That five minute check separates the two and costs nothing.

Type
Check valve cartridge
Fits
Waters 2695 Alliance
Replaces
700000254

Price on request

Enquire

Request a quote
Deuterium lamp for Shimadzu detectors and spectrophotometers, flanged base with a flying lead and a keyed connector.

Spectrophotometer and AAS lamps

Deuterium lamp for Shimadzu UV-Vis spectrophotometers

Deuterium lamp for the Shimadzu UV-Vis bench, cross-referenced to 062-65055-05. These are double beam instruments, which hides lamp ageing for longer than a single beam does: the reference beam absorbs some of the drift, so by the time the baseline looks wrong the lamp is usually well past its useful output. The tell is noise creeping up below 250 nm while the visible range still looks clean. Run the instrument's own energy or lamp history check before ordering, and if the noise covers the whole scan the lamp is not your problem.

Type
Deuterium
Fits
Shimadzu UV-3700, 3600, 2700, 2600, 2550, 2450, 1700, 1800, 1240, 1280
Replaces
062-65055-05
RFID chip
No

Price on request

Enquire

Request a quote
SiliaPrep SPE cartridges, the range of barrel volumes and sorbent weights.

SiliCycle

SiliaPrepX SPE Cartridges, Polymer-Based, HLB

A water wettable polymer, so the bed does not dewet if the cartridge runs dry. That is the commonest reason a silica C18 extraction loses recovery halfway through a batch, and it is the single practical argument for a polymeric sorbent. HLB retains acids, bases and neutrals together, so it is the first choice when you are screening or when the analyte list is mixed. That breadth is also its limit. It holds matrix as readily as it holds analyte, so if you are fighting ion suppression in LC-MS, an ion exchange or mixed mode sorbent will give you a cleaner extract than this one.

Sorbent
HLB
Product line
SiliaPrepX SPE Cartridges, Polymer-Based
Alternative to
Waters Oasis HLB
Particle size
40 µm

CA$202

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Capillary electrophoresis consumables

Capillary electrophoresis consumables

CE-SDS gel buffer

Gel buffer for CE-SDS, cross-referenced to A30341. This is a consumable the assay is validated around rather than one you pick on chromatographic grounds, so the lot and the storage matter more than the price. Sieving performance falls as the gel ages, and a batch stored warm or held too long shows up as migration times that creep and resolution that goes first between the low molecular weight markers. Check the expiry and the storage condition on arrival, and confirm the vial format against your tray before ordering.

Type
Gel buffer
Fits
Beckman CE-SDS
Replaces
A30341

CA$1,379

Enquire

Request a quote
SiliaSep flash chromatography cartridges.

SiliCycle

SiliaSep Flash Cartridges, Silica-Based, C18 (17%) mono

Reversed phase flash on 40 to 63 micron silica, for compounds too polar to separate on bare silica in a normal phase system. It is the flash equivalent of the C18 SPE cartridge and it lets you purify in water and acetonitrile rather than hexane and ethyl acetate, which also makes the fractions easier to hand straight to an LC-MS check. Confirm your flash system can run aqueous mobile phase and that the cartridge fitting matches before ordering.

Sorbent
C18 (17%) mono
Product line
SiliaSep Flash Cartridges, Silica-Based
Particle size
40 - 63 µm
Pore size
60 Å

CA$85

Enquire

Request a quote
SiliaChrom analytical HPLC columns in three lengths.

SiliCycle

SiliaChrom HPLC Column, dt C18

USP L1, and that is the line that matters in a QC lab. A compendial monograph names the packing by L code rather than by brand, so an L1 column of the right dimensions is a legitimate substitution. That gets you in the door. System suitability decides whether it actually runs, because two L1 columns can separate quite differently. Beyond the code, it is built to hold hydrophilic compounds that elute at the solvent front on an ordinary C18, because it takes 100 percent aqueous without the phase collapse. Spherical silica, 99.999 percent pure at 100 Å, so trace metal will not tail your phosphates. Endcapped. One limit: 4.6 mm only, at 3 and 5 micron.

Sorbent
dt C18
Product line
SiliaChrom HPLC Column
Alternative to
Waters Atlantis T3
Pore size
100 Å

CA$1,198

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SCIEX API 4000 triple quadrupole mass spectrometer on a lab cart, with its Leybold turbo pump and backing pumps. used

LC-MS and LC-MS/MS

SCIEX API 4000 LC-MS/MS with ESI source, used

A SCIEX API 4000 triple quadrupole with its ESI source, from a research lab that is clearing equipment. The photographs are the actual instrument, with its turbo pump and the backing pumps on the cart below. It is sold as it stands, so the questions that decide the price are simple: does it pump down, does it pass a tuning check, and which Analyst version runs it. We will check those before we quote and write the answers into the quote. Parts and service for this generation come from the third-party market, and we can help with both. Tell us what you need to run and we will tell you straight whether this is the instrument for it.

Type
Triple quadrupole, LC-MS/MS
Model
API 4000
Ion source
Electrospray (ESI), included
Vacuum
Leybold TURBOVAC TW 701 turbo pump, two rotary backing pumps

Price on request

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Why buy here

Run by a chromatographer,
not a catalogue.

Every distributor can ship you a column. Very few can tell you the one you picked is wrong for a polar analyte, or that your peak is fronting because the injection solvent is stronger than the mobile phase.

Our founder, Dr. Nazmul Alam, spent 14 years running LC-MS and HPLC methods in pharmaceutical, contract research and environmental labs, after a PhD in analytical chemistry at the University of Waterloo in the Pawliszyn group. He reviews every recommendation that leaves Chromkit. The advice comes first. The order follows it.

More about who you're buying from
14
years on LC-MS and HPLC
4
sectors: pharma, CRO, environmental, natural products
18
peer-reviewed publications
200+
chemists and analysts trained

Who you deal with

A chemist checks the recommendation. A service engineer installs and services the instrument.

All people →
Dr. Nazmul Alam

Founder

Dr. Nazmul Alam

Specifies, sources and quotes every order, and answers the technical questions before you buy.

 Syed Hussain

Service partner

Syed Hussain

SPC Analytical, Mississauga

Installs and services the instruments Chromkit sells.

Before you order

Checks worth running, from methods our founder has had to fix.

All guides →

Maintenance

HPLC startup in the morning: prime, wash, then equilibrate

How long to prime depends on how long the system stood and what's in the lines. Degassing, the seal wash, the autosampler prime you shouldn't stop halfway, flow before the lamp, and how long to equilibrate.

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Instruments

Column temperature in HPLC: what the column heater controls, and what it doesn't

Column temperature moves retention time by 1 to 3% per degree. Why 'ambient' isn't a temperature, why the same set point can mean a different column temperature on two systems, and the limits of the column itself.

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Maintenance

How to clean an HPLC detector flow cell, in the right order

A dirty flow cell gives noise, low energy and failed startup tests, the same signs as a weak lamp. How to clean it, mildest solvent first, the low pressure limit that can break it, and what to do when it leaks from inside.

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Not sure which one you need?

Send the analyte and the matrix. We will tell you what to run, then quote it.

Get a quote +1 437-505-5995